Anti-ARF1 (clone AT1B3)

Antibodies, Monoclonal Antibodies
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Product Name Anti-ARF1 (clone AT1B3)
Description ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family member is essential and ubiquitous in eukaryotes, being involved in vesicular transport and functioning as an activator of phospholipase D and cholera toxin. The functions of ARF proteins in membrane traffic and organelle integrity are intimately tied to its reversible association with membranes and specific interactions with membrane phospholipids. ARF1 is various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) localizes in the cytoplasm, while the active form (ARF1-GTP) localizes in the membrane.
Reactivity Human
Applications Dot blot, ELISA, WB
Clone AT1B3. Anti-human ARF1 mAb, clone 1B3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ARF1 protein.
Concentration 0.1 mg/ml
Form Purified, in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide
Species Mouse
Host Mouse
Isotype IgG1, K
Immunogen Recombinant human ARF1 (1-181aa) purified from E. coli
Interest Fields Signal Transduction
NCBI Number NP_ 001649
References Amor, J. C. et al. (1994) Nature 372: 704-708 Claude, A. et al. (1999) J. Cell Biol. 146: 71-84 Derrien, V. et al. (2002) J. Cell Sci. 115: 2867-2879
Storage Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Synonyms ADP-ribosylation factor 1, ADP ribosylation factor 1
Working Dilutions Optimal dilution should be performed by user.

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