| Product Name | Anti-ATG5-ATG12 Complex Antibody |
|---|---|
| Description | Rabbit polyclonal antibody to ATG5-ATG12 Complex. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | Synthetic peptide corresponding to amino acids 262-275 of mouse Atg5, conjugated to Keyhole Limpet Haemocyanin. The corresponding sequence differs by one amino acid in human. |
| Isotype | IgG |
| Molecular Weight | Approximately 56 kDa in mouse liver and human U87 cell lysates. |
| Target | ATG5-ATG12 Complex |
| Amino Acid Sequence | DNFLHISIVPQPTD |
| Protein Length | 14 |
| Specificity | This antibody recognises an epitope within the C-terminal (CT) region of the Atg5-Atg12 complex, which plays a key role in the regulation of autophagy, and is emerging as an important factor in the innate immune response to viruses. The Atg5-Atg12 complex interacts with retinoic acid–inducible gene 1 and the adaptor molecule IFN-beta promoter stimulator 1, through caspase recruitment domains, thereby acting as a negative regulator of the type I IFN production pathway, and as a contributor to RNA viral replication within the host cells (Takeshita et al 2008). |
| Reactivity | Human, Mouse |
| Applications | WB |
| Working Dilutions | WB: 1.0 - 2 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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