| Product Name | Anti-ATRX (Hu) from Mouse (Clone: AX1) |
|---|---|
| Description |
Antibody clone AX1 reacts specifically with ATRX in tissue sections from standard formalin-fixed brain tumor specimens. ATRX is a member of the Snf2 family of helicases/ATPases, which contribute to the remodeling of nucleosome structure. ATRX mutation, IDH1 mutation and chromosomal 1p/19q co-deletion are key molecular factors for the subtype diagnosis of diffuse gli-omas. ATRX mutations in gliomas result in the loss of nuclear ATRX expression, which can be diagnosed by IHC analysis. Loss of ATRX expression is close to being mutually exclusive to 1p/19q co-deletion. Consequently, ATRX immunohistochemistry can be performed to replace laborious analysis of 1p/19q status by FISH techniques. Combined immunohistochemistry of ATRX and IDH1R132H substitutes molecular testing. Astrocytoma very often harbour ATRX-mutations (>90%), wheras Oligodendroglioma typically do not (<5%). The routine practical approach for diagnosing astro-cytomas and oligodendrogliomas begins with perfoming IHC for ATRX and IDH1 R132H expression. Stepwise analysis of mo-lecular parameters with initial IHC for ATRX and IDH1 R132H followed by 1p/19q analysis and then by IDH sequencing signifi-cantly reduces the number of molecular tests required for unequivocal diagnosis (Reuss et al., 2015). |
| Host | Mouse |
| Clone | AX1 |
| Immunogen | Recombinant protein fragment of human ATRX |
| Isotype | IgG1, kappa |
| Specificity | ATRX |
| Reactivity | Human |
| Applications | IHC |
| Form | Lyophilized, in PBS with 2% BSA, 0.05% NaN3, pH 7.4. Antibody purified from culture supernatant. |
| Storage | 2-8°C. For long term storage aliquot and freeze at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Notes | CE IVD |
| Supplier | Dianova GmbH |
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.
