| Product Name | Anti-Aurora B Antibody |
|---|---|
| Description | Rabbit polyclonal antibody to Aurora B. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | Synthetic peptide corresponding to amino acids 227-238 of human Aurora-B kinase. |
| Isotype | IgG |
| Molecular Weight | Approximately 39 kDa in HeLa cell lysates. |
| Target | Aurora B |
| Amino Acid Sequence | SLRRKTMCGTLD |
| Protein Length | 12 |
| Specificity | This antibody recognises human Aurora-B kinase (AurB), a serine/threonine kinase which is the enzymatic core of the Chromosomal Passenger Complex (CPC). This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localisation, stability, and possibly even the substrate specificity of AurB.Two CPCs exist during mitosis- one containing all four members, and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3.AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins. |
| Reactivity | Bovine, Canine, Human, Mouse, Porcine, Primate, Rat |
| Applications | ELISA, WB |
| Working Dilutions | ELISA: 1:2,000 - 1:10,000, WB: 1:250 - 1:1,000 |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide. |
| Concentration | 200 µg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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