| Product Name | Anti-Bad Antibody |
|---|---|
| Description | Rabbit polyclonal antibody to Bad. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | Synthetic peptide of human Bad. |
| Isotype | IgG |
| Molecular Weight | Approximately 18 kDa in PC3 cell lysate under reducing conditions |
| Target | Bad |
| Specificity | This antibody recognises human Bcl2-associated agonist of cell death (BAD), also known as Bcl-xL/Bcl-2-associated death promoter, Bcl-2 binding component 6 or Bcl2-L-8. Similar to the other Bcl-2 protein family members, Bax, Bak, Bid and Bock, BAD has a pro-apoptotic function in the apoptosis signaling cascade.The precise role of BAD in apoptosis signaling depends on BAD’s post-translational modification status. Nonphosphorylated BAD forms heterodimers with the two anti-apoptotic Bcl-2 protein family members, Bcl-xL and Bcl-2. The binding of BAD to Bcl-xL promotes apoptosis by inhibiting the apoptosis repressor function of Bcl-xL. Once serine phosphorylated, BAD is unable to heterodimerize with Bcl-xL and interacts with 14-3-3 proteins instead. This binding retains BAD in the cytoplasm and thereby enables Bcl-xL to suppress apoptosis. |
| Reactivity | Human, Mouse, Rat |
| Applications | IF, IHC-P, WB |
| Working Dilutions | IF: 1:10 - 1:100, IHC-P: 1:50 - 1:200, WB: 1:500 - 1:2,000 |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.02% Sodium Azide. |
| Concentration | Lot Specific |
| Storage | Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Supplier | Antibodies.com |
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