| Product Name | Anti-Bcl-2 Antibody [100] |
|---|---|
| Description | Mouse monoclonal [100] antibody to Bcl-2. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 100 |
| Conjugate | Unconjugated |
| Immunogen | Synthetic peptide corresponding to amino acids 41-54 of the Bcl-2 protein. |
| Isotype | IgG1 |
| Target | Bcl-2 |
| Amino Acid Sequence | GAAPAPGIFSSQPG |
| Protein Length | 14 |
| Specificity | This antibody recognises the human Apoptosis regulator Bcl-2 oncoprotein, a 239 amino acid ~25kDa integral single pass membrane protein containing 4 BH motifs which lies within the cell rather than on the cell surface. The protein is localised in the outer mitochondrial membrane and plays a role in the inhibition of apoptosis. Mouse anti Human Bcl-2, clone 100 reacts with small B lymphocytes in the mantle zone and many cells within T cell areas. Very few cells in germinal centres are stained. In the thymus many cells in the medulla are stained but the cortex shows weak or negative staining. In non-haematopoietic tissues few cells are stained. Mouse anti Human Bcl-2 antibody, clone 100 reacts with neoplastic cells of follicular lymphoma and anaplastic large cell lymphoma (Reed 1997). Mouse anti human Bcl-2, clone 100 has been successfully used to demonstrate Bcl-2 in formalin fixed, paraffin embedded tissues of radioresistant squamous cell carcinoma by immunohistochemistry. |
| Reactivity | Human |
| Applications | IHC-Fr, FC, IHC-P, IP, WB |
| Working Dilutions | FC 1: 1:50 - 1:100, IHC-Fr: 1:20 - 1:40, IHC-P 2: 1:20 - 1:40, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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