| Product Name | Anti-Blood Group Antigen A (CD173), Monoclonal Antibody |
|---|---|
| Description | This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. It is also reactive with the immuno-dominant A trisaccharide. Blood group antigen expression in human colon cancer was studied by means of two monoclonal antibodies of broad anti-A (HE-14) and anti-type 3 and type 4 chain-based A and H (HE-10) specificity. These antigens were proved to re-appear in tumors of the distal colon, the HE-10 antibody reacting more frequently (9 out of 12 samples) than HE-14 (5 out of 12 samples) and frequently with supra-nuclear staining of the cytoplasm probably in those places of the Golgi apparatus where carbohydrate antigens are synthesized. This staining pattern is characteristic of HE-10 in normal colonic mucosa as well. With HE-14, staining was often absent in less differentiated tumors, while HE-10 did react in such tumors. In some cases, these two antibodies gave different staining patterns in parallel sections from the same tissue sample, primarily at the cellular level. Three out of 12 cases showed blood group antigen expression in the mucosa of the distal colon adjacent to the tumor only when HE-10 Ab was used. |
| Synonyms | A transferase, ABO, B transferase, CD173, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Histo-blood group A transferase, Histo-blood group B transferase, NAGAT |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | HE-14 |
| Immunogen | Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A |
| Isotype | IgM, kappa |
| Reactivity | Human |
| Applications | IF, IHC |
| Form | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Gene Id | 28 |
| Uniprot | P16442 |
| Storage | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
| References | Vanðk J, Drðmalovð D, Smyslovð O, N_mec M, Viklickð V, Wisniewski K. Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma. 1989;36(4):479-88 |
| Supplier | ARP |
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