Anti-BrdU Antibody

Category: Antibodies
Catalog
A283414
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Product Name Anti-BrdU Antibody
Description Rabbit polyclonal antibody to BrdU.
Host Rabbit
Clonality Polyclonal
Conjugate Unconjugated
Immunogen Bromodeoxyuridine conjugated to Keyhole Limpet Haemocyanin.
Isotype IgG
Target BrdU
Specificity This antibody recognises bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which when added to cell culture media can be incorporated into the DNA of replicating cells as a substitute for thymidine. This characteristic combined with the ease of detecting incorporated BrdU with the help of anti-BrdU antibodies has resulted in establishing BrdU as a key tool for the visualization of cells in the S-Phase of the cell cycle. BrdU is also commonly used in vivo for monitoring cell proliferation in model systems; BrdU can be administered via intraperitoneal injection, subcutaneous osmotic pump implants or in drinking water.
Reactivity All
Applications FC, ICC, IF, IHC-P
Working Dilutions FC 1: 1:50 - 1:100, ICC: 1:500, IF 2: 1:100 - 1:500, IHC-P: 1:100 - 1:500
Form Liquid
Diluent Buffer Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.
Concentration 1 mg/ml
Storage Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
Supplier Antibodies.com

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