| Product Name | Anti-C3 Antibody [AB01-3B4] |
|---|---|
| Description | Mouse monoclonal [AB01-3B4] antibody to C3. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | AB01-3B4 |
| Conjugate | Unconjugated |
| Immunogen | E. coli derived recombinant human C3 (Gly1200-Asn1663). |
| Isotype | IgG1 |
| Molecular Weight | Approximately 115 kDa and 39 kDa corresponding to the C3 alpha chain and the C3c alpha chain fragment 2 under reducing conditions. Under non-reducing conditions the mature C3 band can be detected at approximately 186 kDa. |
| Target | C3 |
| Amino Acid Sequence | GRLKGPLLNKFLTTAKDKNRWEDPGKQLYNVEATSYALLALLQLKDFDFVPPVVRWLNEQRYYGGGYGSTQATFMVFQALAQYQKDAPDHQELNLDVSLQLPSRSSKITHRIHWESASLLRSEETKENEGFTVTAEGKGQGTLSVVTMYHAKAKDQLTCNKFDLKVTIKPAPETEKRPQDAKNTMILEICTRYRGDQDATMSILDISMMTGFAPDTDDLKQLANGVDRYISKYELDKAFSDRNTLIIYLDKVSHSEDDCLAFKVHQYFNVELIQPGAVKVYAYYNLEESCTRFYHPEKEDGKLNKLCRDELCRCAEENCFIQKSDDKVTLEERLDKACEPGVDYVYKTRLVKVQLSNDFDEYIMAIEQTIKSGSDEVQVGQQRTFISPIKCREALKLEEKKHYLMWGLSSDFWGEKPNLSYIIGKDTWVEHWPEEDECQDEENQKQCQDLGAFTESMVVFGCPN |
| Protein Length | 464 |
| Specificity | This antibody recognises complement C3. C3 is a central component of the classical, alternate and lectin pathways in the complement cascade which forms part of the innate immune defenses. The central position of C3 in the cascade has led to the emergence of immune invasion strategies by many human pathogens, including Staphylococcus aureus which expresses 2 proteins that interfere with the activation of C3. C3 Deficiencies in humans also show an increased susceptibility to bacterial infections in early childhood the development of autoimmune disease and kidney diseases. Conversely, complement over-activation can result in various disease pathologies, including induction of inflammatory pathways causing tissue damage. With complements’ role in various diseases, pharmaceutical and biotech companies have targeted the cascade for potential therapeutic targets in a range of immune and inflammatory diseases as well as in transplantation medicine. C3 is activated via its’ cleavage into fragments which have varying functions within the complement cascade. |
| Reactivity | Human |
| Applications | WB |
| Working Dilutions | WB: 1:1,000, Under reducing conditions the C3 alpha chain at an approximate molecular weight of 115 kDa and the C3c alpha chain fragment 2 at approximately 39 kDa can be detected by WB. Under non reducing conditions the mature C3 band can be detected at a molecular weight of approximately 186 kDa |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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