| Product Name | Anti-C4d Antibody [10-11] |
|---|---|
| Description | Mouse monoclonal [10-11] antibody to C4d. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 10-11 |
| Conjugate | Unconjugated |
| Immunogen | Native C4d from human plasma. |
| Isotype | IgG1 |
| Target | C4d |
| Specificity | This antibody recognises the secreted protein complement component 4d (C4d). The presence of C4d in renal peritubular capillaries is a key indicator for acute antibody-mediated rejection [AMR] (Collins et al. 1999.).C4d was accepted in 2003 into the Banff classification for identification of acute AMR. Mouse anti Human C4d antibody, clone 10-11 is specific for C4d, a marker that can be used in the detection of acute AMR for kidney, heart, pancreas and lung allografts. C4d is regarded as a key marker of antibody-mediated cell injury and humoral rejection. Complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b’s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4b’s short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation. Mouse anti Human C4d antibody, clone 10-11 is used to detect the biomarker C4d which has been described as a “footprint” of antibody mediated tissue rejection. The internal thioester of C4b becomes exposed during cleavage to C4d and forms a covalent bond with the cell surface. The longer half-life of covalently bound C4d makes it a footprint of complement activation long after weakly bound antibodies have been cleared by the blood stream. C4 has also been linked to susceptibility to systemic lupus erythematosus and rheumatoid arthritis. |
| Reactivity | Human |
| Applications | IHC-Fr, ELISA, IF, IHC-P, WB |
| Working Dilutions | ELISA: 1:5,000 - 1:20,000, IF: 1:250 - 1:600, IHC-Fr: 1:100 - 1:750 |
| Form | Liquid |
| Diluent Buffer | Supplied in Borate Buffered Saline with <0.1% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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