| Product Name | Anti-C5 Antibody |
|---|---|
| Description | Sheep polyclonal antibody to C5. |
| Host | Sheep |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | Human C5, purified from plasma. |
| Isotype | IgG |
| Target | C5 |
| Specificity | This antibody recognises complement component 5 (C5), a member of the complement system. The complement system consists of a tightly regulated network of proteins. Its activation leads to a sequential cascade of enzymatic reactions, resulting in opsonisation of the pathogens and their removal by phagocytes, as well as cell lysis. Three pathways have been described, the antibody-dependent pathway (classical pathway), the alternative pathway and the lectin activation pathway (Ehrnthaller 2011, Nesargikar 2012). The complement system plays a critical role in both innate and adaptive immune responses. It is also involved in tissue regeneration, tumor growth and some human pathologies (Sarma 2011). Complement component 5 is the initiator of the effector terminal phase of the complement system. C5 convertase cleaves C5 into C5a, a potent anaphylatoxin, and C5b, ultimately resulting in the formation of the multimeric membrane attack complex (MAC) together with the other components of the terminal pathway (C5b-C9) (Noris 2013). Sheep anti human polyclonal antibody gives a single arc when tested by immunoelectrophoresis (IEP) against human plasma. The identity has been confirmed by double diffusion against fresh human plasma and a known anti human C5. |
| Reactivity | Human |
| Applications | ID, IHC-P |
| Working Dilutions | IHC-P: 1:100 |
| Form | Liquid |
| Diluent Buffer | Supplied in Glycine Buffered Saline with 0.09% Sodium Azide, 0.1% E-amino-n-caproic acid (EACA), 0.01% Benzamidine and 1 mM EDTA. |
| Concentration | Lot Specific |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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