| Product Name | Anti-Caspase-5 Antibody |
|---|---|
| Description | Rabbit polyclonal antibody to Caspase-5. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | A peptide corresponding to 14 amino acids from the centre of human Caspase-5. |
| Isotype | IgG |
| Molecular Weight | Approximately 48 kDa in Ramos cell lysates. (Predicted precursor MWT 47.8 kDa) |
| Target | Caspase-5 |
| Protein Length | 14 |
| Specificity | This antibody recognises an epitope located within the internal region of human Caspase-5, a ~47 kDa cysteine protease, otherwise known as ICH-3. Caspase-5 has been shown to be expressed at higher levels in the colon, lung, spleen, small intestine and by peripheral blood lymphocytes.Caspase-5 is a member of a growing number of inflammatory caspases belonging to the Caspase-1 (ICE-type) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase-1, Caspase-5 is not directly responsible for the activation of IL-1Beta from the pro-IL-1Beta precursor, but the presence of Caspase-5 does appear to enhance the processing of IL-1Beta.The in vivo expression of Caspase-5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma. In vitro cleavage of caspase-5 by the serine protease granzyme B, which plays a major role in cytotoxic T lymphocyte (CTL)-induced apoptosis. |
| Reactivity | Human |
| Applications | WB |
| Working Dilutions | WB: 1 µg/ml - 2 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.02% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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