| Product Name | Anti-Caspase-8 Antibody |
|---|---|
| Description | Rabbit polyclonal antibody to Caspase-8. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | A peptide corresponding to 16 amino acids near the C-terminus of human caspase-8 isoform A. |
| Isotype | IgG |
| Molecular Weight | Detects a cleaved subunit band of approximately 22.5 kDa in Jurkat cell lysates (predicted precursor MWt 57.7 kDa). |
| Target | Caspase-8 |
| Protein Length | 16 |
| Specificity | This antibody recognises an epitope within the C-terminal region (CT) of caspase-8, otherwise known as Fas-linked ICE-like protease (FLICE), which shares homology with both Fas-associated via death domain (FADD) and the ICE/Ced-3 family of cysteine proteases.Caspase-8 is classified as an initiator caspase and acts as the most upstream trigger of Fas-Receptor (CD95) and tumour necrosis factor receptor 1 (TNFR1) mediated apoptosis. Upon activation, the cell death receptor Fas, links with the adaptor molecule FADD via corresponding death domains (DD). FADD in turn then binds via death effector domains (DEDs) to procaspase-8, which then undergoes oligomerization and autocatalytic activation.Active Caspase-8 is capable of cleaving all other known caspases, and also cleaves the Bcl-2 family member BH3-interacting domain death agonist (BID), along with the Bcl-2 associated protein p28Bap31. |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Working Dilutions | WB: 0.5 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.02% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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