| Product Name | Anti-Caspase-9 Antibody |
|---|---|
| Description | Rabbit polyclonal antibody to Caspase-9. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | A peptide corresponding to the internal region of human caspase-9 p10 subunit. |
| Isotype | IgG |
| Molecular Weight | Approximately 47 kDa when run under reducing conditions without induction of apoptosis |
| Target | Caspase-9 |
| Specificity | This antibody recognises caspase-9, a member of the cysteine-aspartic acid protease family. The active form of caspase-9 is generated by cleavage of the pro-caspase-9 protein into fragments, known as the large and small fragments. The trigger for caspase-9 cleavage is the formation of the apoptosome (comprising of cytochrome c, APAF-1, dATP and pro-caspase-9), upon stimulation of the intrinsic apoptosis pathway.The rabbit anti caspase-9 antibody was raised against an internal peptide that is present in one of the small fragments (p10) generated in the pro-caspase-9 cleavage process at Asp330. The antibody will therefore detect pro-caspase-9 (as the motif is present) and the p10 caspase-9 fragment. The antibody can therefore be used to distinguish the p10 caspase-9 fragment from other pro-caspase-9 cleavage products. |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Working Dilutions | WB: 1:200 |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.2% Gelatin and 0.1% Sodium Azide. |
| Concentration | 200 µg/ml |
| Storage | Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use. |
| Supplier | Antibodies.com |
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