| Product Name | Anti-CD11a Antibody [MEM-25] |
|---|---|
| Description | Mouse monoclonal [MEM-25] antibody to CD11a. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-25 |
| Conjugate | Unconjugated |
| Immunogen | Leucocytes from a patient suffering from a LGL-type leukemia. |
| Isotype | IgG1 |
| Target | CD11a |
| Specificity | This antibody recognises the integrin alphaL subunit, a 170-180 kDa type I transmembrane glycoprotein that associates with CD18 to form the leucocyte function-associated antigen-1, an alphaLbeta2 integrin expressed at the surface of all human leukocyte.LFA-1 is a receptor for ICAMs, JAM-1 and F11R. Its interaction with endothelial ligands, such as ICAM-1 and JAM-1 is responsible for leukocyte arrest and extravasation. In addition to its role in leukocyte adhesion and transmigration, LFA-1 is involved in T cell differentiation (Verma et al. 2016, Verman and Kelleher 2017) and is required for the effective lysis of infected cells by cytotoxic T cells (Anikeeva et al. 2005, Pontes Ferreira et al. 2017). LFA-1 also contributes to NK cell cytotoxicity and participates to the clearance of apoptotic neutrophils by macrophages (Kristóf et al. 2013). The interaction of LFA-1 with ICAM-1 is the target of Lifitegrast, a drug approved for the treatment of Dry Eye Disease. Mouse anti Human CD11 antibody, clone MEM-25 inhibits the cytotoxic activity of NK cells. |
| Reactivity | Human |
| Applications | FA, FC, IP |
| Working Dilutions | Flow Cytometry: Neat - 1:50, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide. |
| Concentration | 1 mg/ml |
| Purity | >95% (by SDS-PAGE). |
| Storage | Store undiluted at 4°C. Do not freeze! |
| Supplier | Antibodies.com |
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