Anti-CD13 Antibody [ER-BMDM1]

Category: Antibodies
Catalog
A280354
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Product Name Anti-CD13 Antibody [ER-BMDM1]
Description Rat monoclonal [ER-BMDM1] antibody to CD13.
Host Rat
Clonality Monoclonal
Clone ER-BMDM1
Conjugate Unconjugated
Immunogen BALB/c bone marrow derived macrophages.
Isotype IgG2a
Target CD13
Specificity This antibody recognises the murine homologue of human CD13, a ~160 kDa cell surface antigen with aminopeptidase N activity.In steady state mice, the CD13 antigen is expressed primarily by dendritic cell and mature macrophage subpopulations. The level of CD13 antigen expression increases with maturation, after the monocytic stage. Bone marrow cells and peripheral blood monocytes are reported to be CD13 negative while thioglycollate-elicited macrophages express high levels of CD13.Dendritic cells, interdigitating dendritic cells, veiled cells and Langerhans cells all express CD13, which is reported to be involved in the development of immature dendritic cells from monocytes.
Reactivity Mouse
Applications IHC-Fr, FC
Working Dilutions Flow Cytometry: 1:10 - 1:50, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Form Liquid
Diluent Buffer Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Concentration 1 mg/ml
Storage Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
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