| Product Name | Anti-CD14 Antibody [TÜK4] - BSA and Azide free |
|---|---|
| Description | Mouse monoclonal [TÜK4] antibody to CD14. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | TÜK4 |
| Conjugate | Unconjugated |
| Isotype | IgG2a |
| Target | CD14 |
| Specificity | This antibody recognises the human CD14 cell surface antigen. CD14 is a ~55 kDa glycoprotein that contains multiple leucine-rich repeats. It is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage , a soluble form of CD14 also exists. CD14 is strongly expressed on the surface of monocytes and macrophages but has also been shown to be expressed on the surface of non-myeloid cells (Jersmann 2005). CD14 functions as a pattern recognition receptor (Pugin et al. 1994, Dziarski et al. 1998) in innate immunity for a variety of ligands, in particular for the LPS (endotoxin) of Gram-negative bacteria. Mouse anti human CD14 antibody, clone TÜK4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose –dependent manner. Use of the anti-human CD14 antibody, Low Endotoxin format is recommended for this purpose. |
| Reactivity | Camelus, Bovine, Canine, Feline, Goat, Human, Mink, Porcine, Primate, Rabbit, Sheep |
| Applications | ELISA, FA, FC, WB |
| Working Dilutions | Flow Cytometry: 1:50 - 1:200, Use 5µl of the suggested working dilution to label 106 cells or 100µl whole blood |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline. |
| Concentration | 1 mg/ml |
| Storage | Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Supplier | Antibodies.com |
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