| Product Name | Anti-CD14 Antibody [UCHM1] - BSA and Azide free |
|---|---|
| Description | Mouse monoclonal [UCHM1] antibody to CD14. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | UCHM1 |
| Conjugate | Unconjugated |
| Immunogen | Human Thymocytes followed by peripheral blood mononuclear cells. |
| Isotype | IgG2a |
| Target | CD14 |
| Specificity | This antibody recognises a cell surface antigen of ~55 kDa, known as CD14.The CD14 molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology the CD14 molecule is found to be present on Langerhans cells, follicular dendritic cells, histiocytes and high endothelial venules. Antibodies to the CD14 molecule are known to induce oxidative burst formation. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative. UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver. |
| Reactivity | Fish, Human, Primate |
| Applications | IHC-Fr, FA, FC, IF, IP |
| Working Dilutions | Flow Cytometry: 1:50, Use 10µl of the suggested working dilution to label 106 cells in 100µl. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline. |
| Concentration | 1 mg/ml |
| Storage | Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Supplier | Antibodies.com |
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