| Product Name | Anti-CD16 Antibody [C16/1045] |
|---|---|
| Description | Mouse monoclonal [C16/1045] antibody to CD16. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | C16/1045 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant full-length human CD16 protein. |
| Isotype | IgG1 |
| Molecular Weight | 50-80 kDa |
| Target | CD16 |
| Specificity | It recognizes CD16 (FcgammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80kDa. Two similar genes represent CD16, CD16A (FcgammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (FcgammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation. |
| Reactivity | Human |
| Applications | FC, IF |
| Working Dilutions | Flow Cytometry: 0.5-1 µg/million cells, IF: 0.5-1 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide. |
| Concentration | 200 µg/ml |
| Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Supplier | Antibodies.com |
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