| Product Name | Anti-CD16 / Fc-gamma Receptor III, Monoclonal Antibody |
|---|---|
| Description | Recognizes a protein of 50-65kDa, identified as CD16 (Workshop IV; Code N39 ) (also known low affinity Fc receptor III for IgG (FcRIII) or Leu 11). CD16 exists as a polypepetide-anchored from (FCRIIIA or CD16A) on human natural killer (NK) cells and monocytes/ macrophages and as a glycosylphosphatidylinositol (GPI)-anchored form (FcRIIIB or CD16B) on neutrophils. CD16B is polymorphic and the two alleles are termed NA1 and NA2.3 CD16 plays a role in signal transduction, NK cell activation and antibody-dependent cellular cytotoxicity. This monoclonal antibody has been showed to inhibit the binding of immune complex to NK cells, inhibit cytotoxicity of NK cells, and induce calcium fluxes in NK cells and neutrophils. |
| Synonyms | FcRIIIa, FCR-10, FcRIII, FCGRIII, FcR-10, Fc fragment of IgG, low affinity IIIa, receptor for (CD16), neutrophil-specific antigen NA, CD16a, low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIIIa, immunoglobulin G Fc receptor III, Fc-gamma RIII-alpha, Fc-gamma receptor IIIb (CD16), CD16a antigen, Fc-gamma receptor III-2 (CD 16), FCGR3, Fc gamma receptor III-A, FCG3, low affinity III, receptor for (CD16), Fc-gamma RIII, IGFR3FcgammaRIIIA, CD16FCRIIIA, FCRIII, Fc fragment of IgG, low affinity IIIa, receptor (CD16a) |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | HuNK2 |
| Immunogen | Human peripheral blood lymphocytes from a NK-leukemia patient. |
| Isotype | IgG2a, kappa |
| Reactivity | Human |
| Applications | FA, FC, IF |
| Form | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Gene Id | 2214 |
| Uniprot | P08637 |
| Storage | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
| References | Knapp W. et al. (eds) Leukocyte Typing IV, Oxford University Press, Oxford, 1989.Lanier LL et al. Functional properties of a unique subset of cytotoxic CD3+ T lymphocytes that express Fc receptors for IgG (CD16/Leu-11 antigen). J Exp Med 1985, 162(6):2089-2106.Nagarajan S et al. Ligand binding and phagocytosis by CD16 (Fc gamma receptor III) isoforms. Phagocytic signaling by associated zeta and gamma subunits in Chinese hamster ovary cells. J Biol Chem 1995, 270(43):25762-25770McKenzie SE and Schreiber AD. Biological advances and clinical applications of Fc receptors for IgG. Curr Opin Hematol 1994, 1(1):45-52.Cerboni C et al. CD16-mediated activation of phosphatidylinositol-3 kinase (PI-3K) in human NK cells involves tyrosine phosphorylation of Cbl and its association with Grb2, Shc, pp36 and p85 PI-3K subunit. Eur J Immunol 1998, 28(3):1005-1015.Braakman E et al. CD16 on human ___ðT lymphocytes: expression, function, and specificity for mouse IgG isotypes. Cell Immunol 1992, 143:97-107 |
| Supplier | ARP |
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