| Product Name | Anti-CD161 / NK1.1 Antibody [PK136] |
|---|---|
| Description | Mouse monoclonal [PK136] antibody to CD161 / NK1.1. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PK136 |
| Conjugate | Unconjugated |
| Immunogen | Spleen and bone marrow cells from CE mice. |
| Isotype | IgG2a |
| Target | CD161 / NK1.1 |
| Specificity | This antibody recognises the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family. The NK1.1 surface antigen is also known as CD161b/CD161c and Ly-55.In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc.Although previously thought to recognize only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory. Mouse anti Mouse NK1.1 Antigen antibody, clone PK136 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. Mouse anti Mouse NK1.1 antibody, clone PK136 has also been used for in vivo depletion of NK cells and in vitro activation of NK cells. |
| Reactivity | Mouse |
| Applications | FC, Cytotoxic Assay, IP |
| Working Dilutions | Cytotoxic Assays: 1 µg/ml - 10 µg/ml, Flow Cytometry: 5 µg/ml - 10 µg/ml, Use 10µl of the suggested working dilution to label 1 x 106 cells in 100µl |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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