| Product Name | Anti-CD163 Antibody [ED2] |
|---|---|
| Description | Mouse monoclonal [ED2] antibody to CD163. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ED2 |
| Conjugate | Unconjugated |
| Immunogen | Rat spleen cell homogenate. |
| Isotype | IgG1 |
| Target | CD163 |
| Specificity | This antibody recognises the rat ED2 cell surface glycoprotein. A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor. Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only. Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al. |
| Reactivity | Rat |
| Applications | IHC-Fr, FC, IF, IHC-P, IP, WB |
| Working Dilutions | IHC-Fr: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 500 µg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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