| Product Name | Anti-CD164 Antibody [67D2] (PE) |
|---|---|
| Description | Mouse monoclonal [67D2] antibody to CD164 (PE). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 67D2 |
| Conjugate | PE |
| Immunogen | Breast tumor cell line T-47D. |
| Isotype | IgG1 |
| Target | CD164 |
| Specificity | This antibody recognises CD164, also known as endolyn, a 160 kDa transmembrne homodimer protein. It is composed of an extracellular region and two O-glycosylated mucin domains (I and II). These two domains are linked by a non-mucin cysteine rich subdomain. CD164 is a member of the sialmucin family and is expressed by CD34+ hematopoietic progenitor cells, activated basophils, erythroid cells as well as various cancerous cells. A key role of CD164 is its involvement in regulating adhesion, migration and proliferation of hematopoietic progenitor cells. Elevated expression of CD164 has been observed in patients with Seazy syndrome which is a primary cutaneous T-cell lymphoma and as such, has been suggested as a potential marker of this disease. This clone CD67D2 recognises the class II epitope which is not sensitive to sialidase, N-glycanase and O-sialglycoprotease of CD164. This clones has been used in flow cytometry to examine CD164 upregulation in CD203c+ activated basophils. |
| Reactivity | Human |
| Applications | FC |
| Working Dilutions | Flow Cytometry: Neat, Use 10µl of the undiluted reagent to label 1x106 cells in 100µl |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.2% BSA and <0.1% Sodium Azide. |
| Concentration | Lot Specific |
| Purity | >95% (by SDS-PAGE). |
| Storage | Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. |
| Supplier | Antibodies.com |
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