| Product Name | Anti-CD18a Antibody [PNK-I] (PE) |
|---|---|
| Description | Mouse monoclonal [PNK-I] antibody to CD18a (PE). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PNK-I |
| Conjugate | PE |
| Immunogen | Porcine large granular lymphocytes. |
| Isotype | IgG1 |
| Target | CD18a |
| Specificity | This antibody recognises porcine CD18a. PNK-I was clustered as CD18a at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens. Clone PNK-I immunoprecipitates proteins of ~166 kDa, ~155 kDa and ~95 kDa under non-reducing conditions, specifically recognizing the 95 kDa protein, consistent with the integrin ß2 chain (CD18). PNK-I inhibits porcine NK cell activity independent of any effect on antibody dependent cellular cytotoxicity. CD18 is a single pass type I transmembrane protein and is expressed on all leukocytes and is involved in a variety of cell functions. CD18 acts as a receptor for several ICAM molecules effecting intercellular adhesion functions, it is also involved in the recognition of a variety of extracellular substrate molecules.CD18 acts as a receptor for a number of leukotoxins produced by fungi and bacteria. Clone PNK-I is able to ameliorate the effects of these leukotoxins by blocking binding of the toxins to the CD18 receptor. |
| Reactivity | Camelus, Porcine |
| Applications | FC |
| Working Dilutions | Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl |
| Form | Lyophilized |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide. |
| Concentration | Reconstitution dependent. |
| Storage | Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Supplier | Antibodies.com |
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