| Product Name | Anti-CD27 Antibody [B30C7] |
|---|---|
| Description | Mouse monoclonal [B30C7] antibody to CD27. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | B30C7 |
| Conjugate | Unconjugated |
| Immunogen | Porcine Peripheral Blood Monocytes. |
| Isotype | IgG1 |
| Target | CD27 |
| Specificity | This antibody recognises the porcine homologue of human CD27, previously known as Swine Workshop Cluster 2 (SWC2), a T-cell co-stimulatory molecule belonging to the TNF receptor family. In humans the CD27 antigen is expressed by discrete populations of T- and B-cells where it functions in a co-stimulatory role to induce proliferation of T-cells and B-cells, however, using the B30C7 clone, expression of CD27 on porcine B-cells appears undetectable. Porcine CD27 is expressed by all naïve CD8a-ve T-helper cells and a sub-population of CD8a+ve cells. Monoclonal antibodies to CD27 have previously been used to differentiate between subsets of NK cells and clone B30C7 may be used to differentiate between subsets of pig NK cells. |
| Reactivity | Porcine |
| Applications | FC |
| Working Dilutions | Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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