| Product Name | Anti-CD28 Antibody [YTH913.12] |
|---|---|
| Description | Rat monoclonal [YTH913.12] antibody to CD28. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | YTH913.12 |
| Conjugate | Unconjugated |
| Immunogen | Human Peripheral Blood T cells. |
| Isotype | IgG2b |
| Target | CD28 |
| Specificity | This antibody recognises human CD28, a ~44 kDa single pass type 1 trans-membrane protein expressed as a homodimer on a major subset of human T-cells , responsible for activation of these cells via interaction with the TCR. CD28 is involved in the tuning of the T-cell for activation via TCR, lowering the threshold for activation from around 8000 triggered TCRs to approximately 1500. CD28 along with CD152, also known as CTLA-4 acts as a co-receptor for the co-stimulatory molecules CD80 and CD86. CD28 offers a positive stimulatory role on ligation of CD80 and CD86 while CTLA-4 offers a negative feedback signal preventing CD28 mediated T-cell activation of CD86. Rat anti human CD28, clone YTH913.12 has been reported to recognize an epitope of CD28 expressed by NK cells, which is not recognized by other anti human CD28 clones such as 9.3 and CD28.2 (Galea-Lauri et al 1999.) Other reports however have failed to demonstrate CD28 staining on peripheral blood derived NK cells using clone YTH913.12. |
| Reactivity | Human |
| Applications | IHC-Fr, FC |
| Working Dilutions | Flow Cytometry: 1:25 - 1:50, Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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