| Product Name | Anti-CD3 epsilon Antibody [CD3-12] |
|---|---|
| Description | Rat monoclonal [CD3-12] antibody to CD3 epsilon. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | CD3-12 |
| Conjugate | Unconjugated |
| Immunogen | Synthetic peptide sequence ERPPPVPNPDYEPC from cytoplasmic epitope of CD3. |
| Isotype | IgG1 |
| Target | CD3 epsilon |
| Amino Acid Sequence | ERPPPVPNPDYEPC |
| Specificity | This antibody recognises human CD3e. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (e, ?, d, ?) that assemble and function as three pairs of dimers (e?, ed, ??). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker.As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005). |
| Reactivity | Amphibian, Other, Sea Lion, Gallus, Monkey, Xenopus, Aves, Camelus, Duck, Frog, Bovine, Porpoise, Canine, Equine, Feline, Human, Mouse, Porcine, Primate, Rabbit, Mammal |
| Applications | IHC-Fr, FC, IF, IHC-P, WB |
| Working Dilutions | FC 1: 1:50 - 1:100, IHC-Fr: 1:100, IHC-P 2: 1:100 |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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