| Product Name | Anti-CD4 Antibody [RM4-5] (Amethyst Orange) |
|---|---|
| Description | Rat monoclonal [RM4-5] antibody to CD4 (Amethyst Orange). |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | RM4-5 |
| Conjugate | AO |
| Immunogen | BALB/c mouse thymocytes. |
| Isotype | IgG2a |
| Target | CD4 |
| Specificity | This antibody recognises mouse CD4, a 55 kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance.Rat anti Mouse CD4 antibody, clone RM4-5 can be used for in vitro blocking of ligand binding, as well as in vitro CD4+ T cell depletions. |
| Reactivity | Mouse |
| Applications | FC |
| Working Dilutions | Use 10µl of the suggested working dilution to label 1x106 cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide. |
| Concentration | 100 µg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. |
| Supplier | Antibodies.com |
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