| Product Name | Anti-CD43 Antibody [DFT-1] (FITC) |
|---|---|
| Description | Mouse monoclonal [DFT-1] antibody to CD43 (FITC). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | DFT-1 |
| Conjugate | FITC |
| Immunogen | KG-1 cells. |
| Isotype | IgG1 |
| Target | CD43 |
| Specificity | This antibody recognises human CD43 also known as leukosialin or sialophorin. CD43 is a 381 amino acid single pass type I transmembrane glycoprotein present on the cell surface of multiple cell types including thymocytes, T-lymphocytes, neutrophils, plasma cells and platelets. It is lacking on the surface of most B-lymphocytes. CD34 is a major membrane sialoglycoprotein expressed on T lymphocytes as two predominant glycoforms, a ~115 kDa form present on all T-cells and a ~130 kDa form indicative of T-cell activation and acts as a T-cell counter-receptor for CD169/Siglec-1. Mouse anti Human CD43 antibody, clone DFT-1 binds to both glycoforms of human CD43 CD34 expression is deficient and/or defective in patients suffering from the X-linked chromosomal immunodeficiency condition Wiskott-Aldrich syndrome and acts as a ligand for CD54. Mouse anti human CD43, clone DFT-1 has been used successfully for the evaluation of CD43 expression in patients with Myelodysplastic syndromes. |
| Reactivity | Human |
| Applications | FC |
| Working Dilutions | Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 100µl of whole blood or 106 cells in 100µl. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide. |
| Concentration | 100 µg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. |
| Supplier | Antibodies.com |
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