| Product Name | Anti-CD44 Antibody [156-3C11] |
|---|---|
| Description | Mouse monoclonal [156-3C11] antibody to CD44. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 156-3C11 |
| Conjugate | Unconjugated |
| Immunogen | Stimulated human leucocytes. |
| Isotype | IgG2a |
| Target | CD44 |
| Specificity | This antibody recognises human Phagocytic glycoprotein 1 also known as CD44, HCAM or CD44s. CD44 is a ~90 kDa single pass type I transmembrane glycoprotein. Various isoforms of CD44 exist due to differential expression of exon products form the membrane proximal region of the extracellular domain. recognises the ~90 kDa standard form lacking any of the alternative spliced products, the clone is expected to recognize all isoforms of CD44. CD44 is expressed on leucocytes, erythrocytes, white matter of the brain and some epithelial cells of the breast and small intestine. Antibodies produced by clone 156-3C11 recognise epitope 3, defined as a protease resistant epitope on the CD44 molecule (CD44 and CD45R Cluster report. In Leucocyte Typing V. White cell differentiation antigens. Eds Schlossman, S.F. et al). CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. |
| Reactivity | Monkey, Feline, Human, Primate |
| Applications | IHC-Fr, FC, IF, IHC-P, IP, WB |
| Working Dilutions | Flow Cytometry: 1:50 - 1:100, IHC-Fr: 1:100, IHC-P: 1:100, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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