| Product Name | Anti-CD45 Antibody [F10-89-4] - BSA and Azide free |
|---|---|
| Description | Mouse monoclonal [F10-89-4] antibody to CD45. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | F10-89-4 |
| Conjugate | Unconjugated |
| Immunogen | Human T lymphocytes. |
| Isotype | IgG2a |
| Target | CD45 |
| Specificity | This antibody recognises the human CD45 cell surface antigen, also known as leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Mouse anti Human CD45 antibody, clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. Mouse anti Human CD45 antibody, clone F10-89-4 is routinely tested in flow cytometry on human peripheral blood leucocytes. |
| Reactivity | Human |
| Applications | IHC-Fr, FC, IHC-P, IP |
| Working Dilutions | Flow Cytometry: 1:10 - 1:50, IHC-Fr: 1:500 - 1:1,000, IHC-P: 1:100 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline. |
| Concentration | 1 mg/ml |
| Storage | Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Supplier | Antibodies.com |
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