| Product Name | Anti-CD45 Antibody [IBL-3/16] |
|---|---|
| Description | Rat monoclonal [IBL-3/16] antibody to CD45. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | IBL-3/16 |
| Conjugate | Unconjugated |
| Immunogen | Purified B cells from mouse lymph nodes. |
| Isotype | IgG1 |
| Target | CD45 |
| Specificity | This antibody recognises mouse Receptor-type tyrosine-protein phosphatase C, also known as CD45, Leukocyte common antigen, T200 or Lymphocyte antigen 5 (Ly5). CD45 is a 1291 amino acid ~175kDa single pass type I transmembrane glycoprotein belonging to the protein -tyrosing phosphatase family. CD45 has two fibronectin type-III domains and two tyrosine protein phosphatase domains (UniProt:: P06800).Multiple isoforms are generated by alternative splicing with isoforms having differing deletions in the N-terminal region. Rat anti Mouse CD45 antibody, clone IBL-3/16 is expected to recognize all isoforms of murine CD45.Rat anti Mouse CD45 antibody, clone IBL-3/16 has been used successfully for the identification of CD45 in murine samples using Immunohistochemical (both cryo and FFPE), immunofluorescence and western blotting techniques (Kondo et al. 2011, Cuadros et al. 2006). |
| Reactivity | Mouse |
| Applications | IHC-Fr, FC, IF, IP |
| Working Dilutions | Flow Cytometry: 1:50 - 1:100, IHC-Fr 1: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 106 cells in 100µl |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide. |
| Concentration | 500 µg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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