| Product Name | Anti-CD52 Antibody [YTH34.5] (Amethyst Orange) |
|---|---|
| Description | Rat monoclonal [YTH34.5] antibody to CD52 (Amethyst Orange). |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | YTH34.5 |
| Conjugate | AO |
| Immunogen | Human lymphocytes. |
| Isotype | IgG2b |
| Target | CD52 |
| Specificity | This antibody recognises the human CD52 antigen, also known as CAMPATH-1. The CD52 antigen is a remarkably small but heavily glycosylated peptide attached to the cell surface membrane via a GPI link. The apparent molecular mass of the native antigen on SDS-PAGE is 25-29 kDa, considerably reduced following N-glycanase treatment. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.Humanized versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies (Dearden et al. 2002; Pettitt et al. 2012). |
| Reactivity | Human, Primate |
| Applications | FC |
| Working Dilutions | Cytotoxic Assays: 50 µg/ml (Use human serum as complement source), Flow Cytometry: 1:20, IHC-Fr: 1:20, IHC-P: 1:20 - 1:40 Pack Size: 0.2 mg 1:20 Pack Size: 20 µg, use 10µl of the suggested working dilution to label 1 x 106 cells in 100µl |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide. |
| Concentration | 100 µg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. |
| Supplier | Antibodies.com |
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