| Product Name | Anti-CD53 Antibody [161-2] - BSA and Azide free |
|---|---|
| Description | Mouse monoclonal [161-2] antibody to CD53. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 161-2 |
| Conjugate | Unconjugated |
| Immunogen | Stimulated human leukocytes. |
| Isotype | IgG2a |
| Molecular Weight | 33-55 kDa |
| Target | CD53 |
| Specificity | This antibody recognizes a protein of 33-55kDa, identified as CD53 (Workshop VI; Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This antibody recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1). |
| Reactivity | Human |
| Applications | FC, Functional Studies, IF |
| Working Dilutions | Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free. |
| Concentration | 1 mg/ml |
| Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Supplier | Antibodies.com |
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