Anti-CD55, Decay Accelerating Factor (DAF) Monoclonal Antibody, Biotin
| Product Name | Anti-CD55, Decay Accelerating Factor (DAF) Monoclonal Antibody, Biotin |
|---|---|
| Description | Mouse monoclonal antibody to human CD55, Decay Accelerating Factor (DAF)-Biotin conjugate. CD55 is the complement regulatory protein decay accelerating factor (DAF). It is a 70kDa (in erythrocytes) glycoprotein anchored in the membrane by a glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O- glycans, and also one N- glycan. The amino acid sequence (derived from the cDNA) is known. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens. CD55 was first described on erythrocytes, but is also found on other circulating blood cells3,4. DAF also has a wide distribution on cells in non-haemopoietic tissues, particularly epithelium and endothelium5. CD55 is found specifically at the foetal- maternal interface in placenta6. Soluble forms of DAF are found, for example, in plasma, saliva, urine etc. There are approximately 10,000 CD55 molecules per erythrocyte. CD55 has reduced expression on cells from individuals with paroxysmal nocturnal haemoglobinuria. Individuals with the rare Inab phenotype have an inherited deficiency of CD55. CD55 is also Echo virus7 and Coxackie virus receptor and is a ligand for CD97. |
| Synonyms | DAF, Decay Accelerating Factor, CD55, Echo virus receptor, Coxackie virus receptor |
| Clonality | Monoclonal |
| Clone | BRIC 110 |
| Immunogen | BRIC 110 was made in response to K562 cells. |
| Isotype | Mouse IgG1 kappa light chain |
| Reactivity | Human |
| Form | Biotin-conjugate, in PBS buffer, pH 7.2 containing 0.1% sodium azide, 1% BSA |
| Storage | 2-8C, do not freeze |
| References | 1. Lublin D.M. and Atkinson J.P., (1989) Ann. Rev. Immunol. 7, 35-58 (Review). 2. Daniels G.L. (1989) Vox Sang. 56, 205-211 (Review). 3. Hadam M.R. (1989) in Leucocyte Typing IV; White Cell Differentiation Antigens Ed. W. Knapp et al Oxford University Press pp 694-697. 4. Spring FA et al (1987) Immunology 62 307-313. 5. Anstee DJ and Spring FA (1989) Transfusion Medicine Reviews 3 13-23. 6. Holmes et al., (1990) J. Immunol. 144 3099-3105. 7. Bergelson JM et al (1994) Proc. Natl. Acad. Sci. 91 6245-6248. 8. Tate CG et al. Biochem. J. 1989, 261: 489-493. 9. Coyne KE et al. J. Immunol. 1992, 149: 2906-2913. 10. Klickstein LB and Springer TA (1993) Proceedings of the fifth workshop and conference on white cell differentiation antigens, Boston , vol. 2 p1473-1474. 11. Petty AC et al (1993) Vox Sang 65 309-315. |
| Supplier | ARP |
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