| Product Name | Anti-CD64 Antibody [AT152-9] |
|---|---|
| Description | Rat monoclonal [AT152-9] antibody to CD64. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | AT152-9 |
| Conjugate | Unconjugated |
| Immunogen | HEK293F cells expressing CD64 and gamma chain. |
| Isotype | IgG2a |
| Target | CD64 |
| Specificity | This antibody recognises mouse CD64 also known as FcRI. CD64 is a high affinity activatory receptor for IgG2a and a low affinity receptor for IgG2b and IgG3 type antibodies. The interaction between Fc receptors and antibodies play important roles in both the innate and adaptive immune responses.CD64, through binding of the Fc segment of IgG, mediates phagocytosis and plays a role in antibody-dependent cellular cytotoxicity and clearance of immune complexes. In addition, CD64 also functions as an antigen capture for presentation to T-cells and also mediates the release of cytokines and reactive oxygen intermediates including interleukin (IL)-1, IL-6 and tumor necrosis factor (TNF) alpha. It is constitutively expressed on monocytes and macrophages, germinal centre dendritic cells and early myeloid lineage cells, but not lymphocytes. Expression on monocytes can be strongly upregulated by treatment with interferon (IFN) gamma or G-CSF, and can be induced on neutrophils and eosinophils by IFN gamma. |
| Reactivity | Mouse |
| Applications | IHC-Fr, FC |
| Working Dilutions | Flow Cytometry: 1:10, IHC-Fr: 1:1,000, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl |
| Form | Liquid |
| Diluent Buffer | Supplied in TRIS Buffered Saline with <0.1% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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