| Product Name | Anti-CD69 Antibody [FN50] |
|---|---|
| Description | Mouse monoclonal [FN50] antibody to CD69. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | FN50 |
| Conjugate | Unconjugated |
| Immunogen | Activated human B-cells. |
| Isotype | IgG1 |
| Target | CD69 |
| Specificity | This antibody recognises the human early activation antigen CD69, also known as activation inducer molecule (AIM), Early T-cell activation antigen p60, EA1 or MLR-3. CD69 is a 199 amino acid single pass type II transmembrane glycoprotein of ~30 kDa containing a single C-type lectin domain and a single potential N-glycosylation site. CD69 is expressed as a disulphide bond linked homodimer of ~60 kDa (López-Cabrera et al. 1993).CD69 is a marker of early activation expressed by B and T lymphocytes, natural killer cells(Werfel 1997), neutrophils, thymocytes and platelets. Expression of CD69 is rapidly induced on activation by infection or chronic inflamation. Multiple dimeric glycoforms of CD69 can be formed through differential glycosylation of the monomeric subunits. Mouse anti Human CD69 , clone FN50 is useful for the detection of CD69 by flow cytometry and immunohistochemistry on frozen tissue sections. |
| Reactivity | Human, Primate |
| Applications | IHC-Fr, FC, IF |
| Working Dilutions | Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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