| Product Name | Anti-CD86 Antibody [BU63] |
|---|---|
| Description | Mouse monoclonal [BU63] antibody to CD86. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | BU63 |
| Conjugate | Unconjugated |
| Immunogen | Human peripheral blood lymphocytes. |
| Isotype | IgG1 |
| Target | CD86 |
| Specificity | This antibody recognises human CD86 also known as B7-2, a type I transmembrane protein expressed by monocytes and activated B cells. CD86 acts as a co-stimulaory molecule along with CD80 and is a ligand for CD28 and CTLA-4. CD86 is a member of the Immunoglobulin superfamily and carries an extracellular domain bearing both an Ig-v-like domain which contains the CTLA-4 binding site and an adjacent C2-like domain. CD86 plays an important role in co-stimulation of T cell proliferation , IL-2 production and in the primary immune response. Domain depletion epitope mapping indicates that the binding site of Mouse anti Human CD86,clone Bu63 is located within the Ig-v-like domain of human CD86. CD86 along with CD80 may be exploited as receptors for adenovirus entry into cells (Short et al. 2004 2006). |
| Reactivity | Human |
| Applications | IHC-Fr, FC, IP |
| Working Dilutions | Flow Cytometry: 1:20 - 1:100, IHC-Fr: 1:20 - 1:100, Use 10µl of the suggested working dilution to label 106 cells in 100µl. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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