| Product Name | Anti-CDK1 Antibody |
|---|---|
| Description | Rabbit polyclonal antibody to CDK1. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | A peptide corresponding to the C-terminus of human Cdc2. |
| Isotype | IgG |
| Molecular Weight | Approximately 34 kDa when run under reducing conditions |
| Target | CDK1 |
| Specificity | This antibody recognises cell division control protein 2 homolog (Cdc2) also known as cyclin-dependent kinase 1 (CDK1) and p34 protein kinase. Cdc2, a serine/threonine kinase, plays a crucial role in regulation of the eukaryotic cell cycle by phosphorylating proteins involved in DNA replication and other important cell cycle progression processes. Binding of cyclins to Cdc2 is essential for its regulation, and the kinase only becomes activated once bound to cyclins. Cyclin binding has also been reported to play a role in substrate recruitment. Cdc2 activity is highly regulated through phosphorylation by the serine/threonine protein kinase Wee1. This phosphorylation results in Cdc2 inactivation and consequently, cell cycle arrest at the G2/M transition stage. Wee1 phosphorylation is counteracted by the Cdc25 phosphatase, which dephosphorylates Cdc2 thereby enabling entry into mitosis. |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Working Dilutions | WB: 1:400 |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.2% Gelatin and 0.1% Sodium Azide. |
| Concentration | 200 µg/ml |
| Storage | Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use. |
| Supplier | Antibodies.com |
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