| Product Name | Anti-CHEK2 Antibody [PCRP-CHEK2-1A4] |
|---|---|
| Description | Mouse monoclonal [PCRP-CHEK2-1A4] antibody to CHEK2. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PCRP-CHEK2-1A4 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant fragment of human CHEK2 corresponding to protein domain |
| Isotype | IgG2b |
| Molecular Weight | 60.91 kDa |
| Target | CHEK2 |
| Specificity | This antibody recognizes a serine/threonine protein kinase that is a required check-point to mediate cell cycle arrest, activation of DNA repair and apoptosis. In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. Also, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. |
| Reactivity | Human |
| Applications | FC, IF, IP, WB |
| Working Dilutions | IP: 1-2µg / 100-500µg proteins, Flow Cytometry: 1-2 µg/million cells, WB: 1-2 µg/ml, IF: 1-2 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide. |
| Concentration | 200 µg/ml |
| Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Supplier | Antibodies.com |
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