| Product Name | Anti-Clostridium difficile Toxin A Antibody [253/422] |
|---|---|
| Description | Mouse monoclonal [253/422] antibody to Clostridium difficile Toxin A. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 253/422 |
| Conjugate | Unconjugated |
| Immunogen | C. difficile Toxin A. |
| Isotype | IgG1 |
| Molecular Weight | 308 kDa (Toxin A) |
| Target | Clostridium difficile Toxin A |
| Specificity | This antibody reacts with C. difficile Toxin A, but not with Cholera subunit a, Cholera toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. Clostridium difficile is a major nosocomial pathogen that causes antibiotic- associated colitis. Clostridium difficile mediates inflammatory diarrhea by releasing two large protein enterotoxins (Toxin A and Toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. Clostridium difficile Toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. Clostridium difficile Toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation. |
| Reactivity | Bacteria |
| Applications | ELISA, IF |
| Working Dilutions | ELISA: 1-5 µg/ml, IF: 1-2 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide. |
| Concentration | 200 µg/ml |
| Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Supplier | Antibodies.com |
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