| Product Name | Anti-CTLA4 Antibody [1B8] |
|---|---|
| Description | Hamster monoclonal [1B8] antibody to CTLA4. |
| Host | Hamster |
| Clonality | Monoclonal |
| Clone | 1B8 |
| Conjugate | Unconjugated |
| Immunogen | Extracellular portion of mouse CD152 fused to a mouse IgG2a. |
| Isotype | IgG |
| Target | CTLA4 |
| Specificity | This antibody recognises mouse CD152, commonly known as CTLA-4 (cytotoxic T-lymphocyte protein 4), a structural homolog for CD28. CD152 binds the same ligands as CD28, namely CD80 (B7.1) and CD86 (B7.2), but with much higher affinity. However, while binding of CD28 enhances T cell function, binding of CD152 inhibits it. CD152 is not detected on naïve T-cells and is induced upon T-cell activation. In mice, lack of CD152 leads to massive lymphoproliferative disorder leading to tissue destruction and autoimmunity, suggesting a critical role for CD152 in limiting T-cell responses. CD152 has been shown to reduce IL-2 production after ligation and can inhibit T-cell expansion. This regulatory role and the high binding affinity of CD152 make it a potential therapy for autoimmune diseases such as rheumatoid arthritis. Hamster anti Mouse CD152 antibody, clone 1B8 may be used to enhance proliferation and pro-inflammatory cytokines of lymph node cells. It may also be used to enhance tumour immunity. |
| Reactivity | Mouse |
| Applications | ELISA, FC, IF, IP, WB |
| Working Dilutions | Flow Cytometry: 1:10 - 1:100, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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