| Product Name | Anti-Cyclin B1 (G2- & M-phase Cyclin), Monoclonal Antibody |
|---|---|
| Description | It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. |
| Synonyms | CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CCNB1/1098 |
| Immunogen | Recombinant human full-length CCNB1 protein |
| Isotype | IgG1, kappa |
| Reactivity | Human, Mouse |
| Applications | FC, IF, IHC |
| Form | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Gene Id | 891 |
| Uniprot | P14635 |
| Storage | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
| References | Galaktionov, K. and Beach D. 1991. Specific activation of Cdc25 tyrosine phosphatases by B type cyclins: Evidence for multiple roles of mitotic cyclins. Cell 67: 1181-1194 |
| Supplier | ARP |
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