| Product Name | Anti-Cytochrome b245 Light Chain / p22-phox Antibody [CS9] |
|---|---|
| Description | Mouse monoclonal [CS9] antibody to Cytochrome b245 Light Chain / p22-phox. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CS9 |
| Conjugate | Unconjugated |
| Immunogen | Cytochrome B245 solubilised in octyl-beta-gluycopyranoside. |
| Isotype | IgG1 |
| Molecular Weight | Approximately 19 kDa in neutrophil membrane extracts. |
| Target | Cytochrome b245 Light Chain / p22-phox |
| Specificity | This antibody recognises the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections. The light chain (p22phox), along with the heavy chain (gp91phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons. The core region of the CS9 epitope is amino acids 165 - 169 (KKPSE), a cytosolic epitope on p22phox not accessible on intact neutrophils. Mouse anti Human Cytochrome B245 Light Chain antibody, clone CS9 also has an inhibitory effect on NADPH oxidase activation. |
| Reactivity | Human |
| Applications | IHC-Fr, ELISA, FC, IP, WB |
| Working Dilutions | FC 1: 1:10 - 1:100, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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