| Product Name | Anti-Cytokeratin 10 + 13 Antibody [SPM262] |
|---|---|
| Description | Mouse monoclonal [SPM262] antibody to Cytokeratin 10 + 13. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SPM262 |
| Conjugate | Unconjugated |
| Immunogen | Cytoskeletal preparation extracted from human ectocervical epithelium. |
| Isotype | IgG2a |
| Molecular Weight | 56.5 kDa (CK10) / 53 kDa (CK13) |
| Target | Cytokeratin 10 + 13 |
| Specificity | This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. |
| Reactivity | Feline, Human |
| Applications | FC, IF, IHC-P, WB |
| Working Dilutions | Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide. |
| Concentration | 200 µg/ml |
| Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Supplier | Antibodies.com |
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