| Product Name | Anti-DARPP32 (phospho Thr34) Antibody |
|---|---|
| Description | Rabbit polyclonal antibody to DARPP32 (phospho Thr34). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | Synthetic phosphopeptide corresponding to an amino acid sequence within DARPP-32 which includes phosphorylated Thr34. |
| Isotype | IgG |
| Molecular Weight | Approximately 32 kDa in rat caudate lysates. |
| Target | DARPP32 |
| Specificity | This antibody recognises DARPP-32, also known as protein phosphatase 1 regulatory subunit 1B and dopamine- and cAMP-regulated neuronal phosphoprotein, when phosphorylated at threonine 34. DARPP-32 is a 205 amino acid ~32 kDa member of the protein phosphatase inhibitor 1 family.DARPP-32 is principally expressed in striatal medium spiny neurons, and plays a critical role in the regulation of dopaminergic neurotransmission.DARPP-32 can act either as a phosphatase inhibitor or as a kinase inhibitor, depending on its relative state of phosphorylation. Phosphorylation at threonine 34 converts DARPP-32 into an inhibitor of protein phosphatase-1 (PP-1) whilst phosphorylation at threonine 75 switches the protein to an inhibitor of protein kinase A (PKA). G-protein coupled receptor 6 deficiency in a mouse model of Parkinsons disease leads to an increase in DARPP-32 (pThr34) in striatopalladial neurons with a concommitent increase in locomotor activity and reduced abnormal movements in the mouse dyskinesia model of Parkinson's disease, thus suggesting treatment other than dopamine replacement for the condition. |
| Reactivity | Gallus, Monkey, Bovine, Canine, Human, Mouse, Rat |
| Applications | WB |
| Working Dilutions | WB: 1:1,000 |
| Form | Liquid |
| Diluent Buffer | Supplied in 10mM HEPES, pH 7.5, with 0.1% BSA, 50% Glycerol, and 0.09% Sodium Azide. |
| Concentration | Lot Specific |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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