| Product Name | Anti-DEP1 Antibody [MEM-CD148/05] (PE) |
|---|---|
| Description | Mouse monoclonal [MEM-CD148/05] antibody to DEP1 (PE). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-CD148/05 |
| Conjugate | PE |
| Immunogen | Human recombinant CD148 (amino acids 1-444). |
| Isotype | IgG2b |
| Target | DEP1 |
| Amino Acid Sequence | MKPAAREARLPPRSPGLRWALPLLLLLLRLGQILCAGGTPSPIPDPSVATVATGENGITQISSTAESFHKQNGTGTPQVETNTSEDGESSGANDSLRTPEQGSNGTDGASQKTPSSTGPSPVFDIKAVSISPTNVILTWKSNDTAASEYKYVVKHKMENEKTITVVHQPWCNITGLRPATSYVFSITPGIGNETWGDPRVIKVITEPIPVSDLRVALTGVRKAALSWSNGNGTASCRVLLESIGSHEELTQDSRLQVNISGLKPGVQYNINPYLLQSNKTKGDPLGTEGGLDASNTERSRAGSPTAPVHDESLVGPVDPSSGQQSRDTEVLLVGLEPGTRYNATVYSQAANGTEGQPQAIEFRTNAIQVFDVTAVNISATSLTLIWKVSDNESSSNYTYKIHVAGETDSSNLNVSEPRAVIPGLRSSTFYNITVCPVLGDIEGT |
| Protein Length | 444 |
| Specificity | This antibody recognises CD148, a member of the receptor protein tyrosine phosphatase family. CD148 contains an ectodomain composed of multiple fibronectin type III-like repeats, a transmembrane domain and a single catalytic phosphatase domain. CD148 is broadly expressed including in platelets, T cells, B cells, macrophages as well as in some non-hematopoietic cells. CD148 functions as a key regulator of platelet reactivity via its regulation of Src family kinases (SFKs). Deletion of CD148 in mice has been shown to significantly impact platelet reactivity and thrombosis via SFK activity regulation. Clone MEM-CD148/05 has been used in immunofluorescence experiments to examine the co-localization of CD148 with CD45 at the immunological synapses formed between Tim-3hiCD8+ T cells. |
| Reactivity | Human |
| Applications | FC |
| Working Dilutions | Flow Cytometry: Neat, Use 10µl of the undiluted reagent to label 1x106 cells in 100µl |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.2% BSA and <0.1% Sodium Azide. |
| Concentration | Lot Specific |
| Purity | >95% (by SDS-PAGE). |
| Storage | Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. |
| Supplier | Antibodies.com |
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