| Product Name | Anti-DLL4 Antibody |
|---|---|
| Description | Rabbit polyclonal antibody to DLL4. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | Synthetic peptide corresponding to the internal region of human DLL4. |
| Isotype | IgG |
| Molecular Weight | Approximately 74 kDa in pancreatic cell lysates. |
| Target | DLL4 |
| Specificity | This antibody recognises human Delta-like protein 4 (DLL4), also known as Delta-4. DLL4 is a 685 amino acid single pass type 1 transmembrane glycoprotein of ~72 kDa containing a single DSL domain and eight EGF-like domains. Human DLL4 is the homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the Notch receptor, Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formation of a differentiated vascular network. DLL4 is strongly expressed in tumor vessels of primary renal tumors , bladder cancer , and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumor growth, with no apparent toxicity. Expression of DLL4 in normal human dermis is low in foetal tissues, becomes more intense during early life (0-20 years) and gradually declines thereafter as shown by immunohistochemisrtry on FFPE tissues using rabbit anti human DLL4 antibody. |
| Reactivity | Human, Mouse, Primate, Rat |
| Applications | ELISA, IHC-P, WB |
| Working Dilutions | ELISA: 1:3,000 - 1:15,000, IHC-P: 5 - 15 µg/ml, WB: 1:300 - 1:1500 |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide. |
| Concentration | 500 µg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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