| Product Name | Anti-EIF4E Antibody [PCRP-EIF4E-1D3] - BSA and Azide free |
|---|---|
| Description | Mouse monoclonal [PCRP-EIF4E-1D3] antibody to EIF4E. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PCRP-EIF4E-1D3 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant full-length human EIF4E protein. |
| Isotype | IgG2a |
| Molecular Weight | 25.1 kDa |
| Target | EIF4E |
| Specificity | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4E, eIF4A and eIF4G. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA cap during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A and the eIF3 complex. |
| Reactivity | Human |
| Applications | FC, IF, WB |
| Working Dilutions | Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free. |
| Concentration | 1 mg/ml |
| Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Supplier | Antibodies.com |
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