| Product Name | Anti-eIF4EBP1 Antibody |
|---|---|
| Description | Rabbit polyclonal antibody to eIF4EBP1. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | A peptide corresponding to 14 amino acids near human 4EBP1 carboxy-terminus. |
| Isotype | IgG |
| Molecular Weight | Approximately 20 kDa in mouse 3T3 cell lysates. (predicted Mwt. 13 kDa). |
| Target | eIF4EBP1 |
| Protein Length | 14 |
| Specificity | This antibody recognises an epitope within the C-terminal region (CT) of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1), a rapamycin-sensitive downstream target of TOR (Target of rapamycin), and regulatory factor of eukaryotic mRNA translation.The association between 4EPB1 and eukaryotic translation initiation factor 4E (eIF-4E) inhibits the binding of eIF-4E with p220, thereby preventing the formation of a functional eIF-4E complex, essential for mRNA translation. Phosphorylation of 4EBP1 following stimulation by mitogens such as insulin, results in the release of eIF-4E from 4EBP1, thereby enabling the translation complex to assemble.Rabbit anti 4EBP1 antibody (AHP993) has been reported as suitable for use in IHC on mouse 3T3 cell cytospins. |
| Reactivity | Human, Mouse |
| Applications | WB |
| Working Dilutions | WB: 2.5 - 5.0 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.02% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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